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Antibody Product Overview
| ELK.No | ES3091 |
| Product name | p115 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | USO1; VDP; General vesicular transport factor p115; Protein USO1 homolog; Transcytosis-associated protein; TAP; Vesicle-docking protein |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 8615 |
| Human Swiss-Prot | O60763 |
| Source | Rabbit |
| Isotype | IgG |
| Target | p115 |
| Fields | |
| Gene name | USO1 |
| Protein name | General vesicular transport factor p115 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q9Z1Z0 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human USO1. AA range:913-962 |
| Specificity | p115 Polyclonal Antibody detects endogenous levels of p115 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 108kD |
| Background | The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014], |
| Function | domain:Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.,function:General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.,PTM:Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.,similarity:Belongs to the VDP/USO1/EDE1 family.,subcellular location:Recycles between the cytosol and the Golgi apparatus during interphase., |
| Subcellular location | Cytoplasm, cytosol . Golgi apparatus membrane ; Peripheral membrane protein . Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes. . |
| Expression | Epithelium,Human skeletal muscle,Liver,Uterus, |
Additional Images
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Western Blot analysis of various cells using p115 Polyclonal Antibody diluted at 1:2000
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Western Blot analysis of K562 cells using p115 Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from MCF-7, HeLa, and Jurkat cells, using USO1 Antibody. The lane on the right is blocked with the synthesized peptide.
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: AO-06-ES3091-100
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