Français
English
Antibody Product Overview
| ELK.No | ES3184 |
| Product name | PFK-2 liv/tes rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | PFKFB1; F6PK; PFRX; 6-phosphofructo-2-kinase/fructose-2; 6-bisphosphatase 1; 6PF-2-K/Fru-2,6-P2ase 1; PFK/FBPase 1; 6PF-2-K/Fru-2,6-P2ase liver isozyme; PFKFB4; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4; 6PF-2-K/Fru-2,6-P2ase 4; |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 5207/5210 |
| Human Swiss-Prot | P16118/Q16877 |
| Source | Rabbit |
| Isotype | IgG |
| Target | PFK-2 liv/tes |
| Fields | >>Fructose and mannose metabolism;>>Metabolic pathways;>>AMPK signaling pathway;>>Glucagon signaling pathway |
| Gene name | PFKFB1/PFKFB4 |
| Protein name | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1/4 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 18639/270198 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | 24638/54283 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P07953/P25114 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PFKFB1/4. AA range:331-380 |
| Specificity | PFK-2 liv/tes Polyclonal Antibody detects endogenous levels of PFK-2 liv/tes protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 54kD |
| Background | This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012], |
| Function | catalytic activity:ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.,catalytic activity:Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate.,enzyme regulation:Phosphorylation results in inhibition of the kinase activity.,function:Synthesis and degradation of fructose 2,6-bisphosphate.,similarity:In the C-terminal section; belongs to the phosphoglycerate mutase family.,subunit:Homodimer.,tissue specificity:Liver., |
| Subcellular location | cytosol,6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex, |
| Expression | Liver. |
Additional Images
No image
Western Blot analysis of various cells using PFK-2 liv/tes Polyclonal Antibody
No image
Western blot analysis of lysates from HeLa, HepG2, COLO205, and 293 cells, using PFKFB1/4 Antibody. The lane on the right is blocked with the synthesized peptide.
No image
Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
No image
: AO-06-ES3184-50
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package