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Antibody Product Overview
| ELK.No | ES3263 |
| Product name | PRPF18 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | PRPF18; HPRP18; Pre-mRNA-splicing factor 18; PRP18 homolog; hPRP18 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 8559 |
| Human Swiss-Prot | Q99633 |
| Source | Rabbit |
| Isotype | IgG |
| Target | PRPF18 |
| Fields | >>Spliceosome |
| Gene name | PRPF18 |
| Protein name | Pre-mRNA-splicing factor 18 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 67229 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8BM39 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 171552 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q9JKB8 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PRPF18. AA range:182-231 |
| Specificity | PRPF18 Polyclonal Antibody detects endogenous levels of PRPF18 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 40kD |
| Background | Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. [provided by RefSeq, Jul 2008], |
| Function | function:Participates in the second step of pre-mRNA splicing.,similarity:Belongs to the PRP18 family.,subcellular location:Colocalizes with spliceosomal snRNPs.,subunit:Heterodimer with PPIH. Interacts with PRPF4 and with the spliceosome. Part of a complex containing U4/U6 snRNPs., |
| Subcellular location | Nucleus speckle . Colocalizes with spliceosomal snRNPs. . |
| Expression | Brain,Cervix carcinoma,Placenta, |
Additional Images
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Western Blot analysis of various cells using PRPF18 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western Blot analysis of HuvEc cells using PRPF18 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of lysates from 293, MCF-7, and HUVEC cells, using PRPF18 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES3263-50
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