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Ribosomal Protein S12 rabbit pAb
AO-06-ES3370-50
Antibody Product Overview
| ELK.No | ES3370 |
| Product name | Ribosomal Protein S12 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | RPS12; 40S ribosomal protein S12 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 6206 |
| Human Swiss-Prot | P25398 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Ribosomal Protein S12 |
| Fields | >>Ribosome;>>Coronavirus disease - COVID-19 |
| Gene name | RPS12 |
| Protein name | 40S ribosomal protein S12 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | P63323 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | P63324 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RPS12. AA range:83-132 |
| Specificity | Ribosomal Protein S12 Polyclonal Antibody detects endogenous levels of Ribosomal Protein S12 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 15kD |
| Background | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S12E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal cancers compared to matched normal colonic mucosa has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008], |
| Function | similarity:Belongs to the ribosomal protein S12e family., |
| Subcellular location | Cytoplasm. |
| Expression | Cervix carcinoma,Embryonic stem cell,Hepatoma,Mammary carcinoma,Placenta,Skin,Testi |
Additional Images
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Western Blot analysis of various cells using Ribosomal Protein S12 Polyclonal Antibody
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of A549 cells, using RPS12 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COLO cells, using RPS12 Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES3370-50
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