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Antibody Product Overview
| ELK.No | ES3382 |
| Product name | M-RIP rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | MPRIP; KIAA0864; MRIP; RHOIP3; Myosin phosphatase Rho-interacting protein; M-RIP; Rho-interacting protein 3; RIP3; p116Rip |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 23164/23164 |
| Human Swiss-Prot | Q6WCQ1 |
| Source | Rabbit |
| Isotype | IgG |
| Target | M-RIP |
| Fields | |
| Gene name | MPRIP |
| Protein name | Myosin phosphatase Rho-interacting protein |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 26936 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P97434 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 116504 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q9ERE6 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from the Internal region of human M-RIP. |
| Specificity | M-RIP Polyclonal Antibody detects endogenous levels of M-RIP protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 115kD |
| Background | function:Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.,similarity:Contains 1 PH domain.,similarity:Contains 2 PH domains.,subcellular location:Colocalizes with F-actin.,subunit:Binds F-actin through its N-terminus (By similarity). Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains., |
| Function | function:Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.,similarity:Contains 1 PH domain.,similarity:Contains 2 PH domains.,subcellular location:Colocalizes with F-actin.,subunit:Binds F-actin through its N-terminus (By similarity). Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains., |
| Subcellular location | Cytoplasm, cytoskeleton . Colocalizes with F-actin. |
| Expression | Aorta,Brain,Epithelium,Lymph,Melanoma,Muscle,Ovary,Tongue, |
Additional Images
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Western Blot analysis of various cells using M-RIP Polyclonal Antibody
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Western Blot analysis of 293 cells using M-RIP Polyclonal Antibody
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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES3382-50
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