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Antibody Product Overview
| ELK.No | ES3383 |
| Product name | Rit1 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA;IHC |
| Other name | RIT1; RIBB; RIT; ROC1; GTP-binding protein Rit1; Ras-like protein expressed in many tissues; Ras-like without CAAX protein 1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 6016 |
| Human Swiss-Prot | Q92963 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Rit1 |
| Fields | |
| Gene name | RIT1 |
| Protein name | GTP-binding protein Rit1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 19769 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P70426 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RIT1. AA range:170-219 |
| Specificity | Rit1 Polyclonal Antibody detects endogenous levels of Rit1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 25kD |
| Background | This gene encodes a member of a subfamily of Ras-related GTPases. The encoded protein is involved in regulating p38 MAPK-dependent signaling cascades related to cellular stress. This protein also cooperates with nerve growth factor to promote neuronal development and regeneration. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012], |
| Function | enzyme regulation:Alternate between an inactive form bound to GDP and an active form bound to GTP.,function:Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation.,miscellaneous:Shows rapid uncatalyzed guanine nucleotide dissociation rates, which are much faster than those of most Ras subfamily members.,miscellaneous:Stimulation of the NGF and EGF receptor signaling pathways results in rapid and prolonged activation.,similarity:Belongs to the small GTPase superfamily. Ras family.,subunit:Interacts with MLLT4, the C-terminal domain of RALGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form. Strongly interacts with BRAF, but only weakly with RAF1. BARF and RAF1 association is dependent upon the GTP-bound state. Interacts with RGL3.,tissue specificit |
| Subcellular location | Cell membrane. |
| Expression | Expressed in many tissues. |
Additional Images
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Western Blot analysis of various cells using Rit1 Polyclonal Antibody diluted at 1:500
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Western blot analysis of lysates from Jurkat cells, using RIT1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES3383-100
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