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Antibody Product Overview
| ELK.No | ES3400 |
| Product name | RUNX3 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA |
| Other name | RUNX3; AML2; CBFA3; PEBP2A3; Runt-related transcription factor 3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; CBF-alpha-3; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEA2-alpha C; PEB |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 864 |
| Human Swiss-Prot | Q13761 |
| Source | Rabbit |
| Isotype | IgG |
| Target | RUNX3 |
| Fields | >>Th1 and Th2 cell differentiation;>>Epstein-Barr virus infection |
| Gene name | RUNX3 |
| Protein name | Runt-related transcription factor 3 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q64131 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RUNX3. AA range:133-182 |
| Specificity | RUNX3 Polyclonal Antibody detects endogenous levels of RUNX3 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 44kD |
| Background | This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016], |
| Function | domain:A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.,function:CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.,similarity:Contains 1 Runt domain.,subunit:Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1., |
| Subcellular location | Nucleus . Cytoplasm . The tyrosine phosphorylated form localizes to the cytoplasm. Translocates from the cytoplasm to the nucleus following TGF-beta stimulation. . |
| Expression | Expressed in gastric cancer tissues (at protein level). |
Additional Images
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Western Blot analysis of various cells using RUNX3 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of lysate from HUVEC cells, using RUNX3 antibody.
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: AO-06-ES3400-50
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