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Antibody Product Overview
| ELK.No | ES3404 |
| Product name | SAE1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | SAE1; AOS1; SUA1; UBLE1A; SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 10055 |
| Human Swiss-Prot | Q9UBE0 |
| Source | Rabbit |
| Isotype | IgG |
| Target | SAE1 |
| Fields | >>Ubiquitin mediated proteolysis |
| Gene name | SAE1 |
| Protein name | SUMO-activating enzyme subunit 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 56459 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9R1T2 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 308384 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q6AXQ0 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AOS1. AA range:221-270 |
| Specificity | SAE1 Polyclonal Antibody detects endogenous levels of SAE1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 38kD |
| Background | SUMO1 activating enzyme subunit 1(SAE1) Homo sapiens Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010], |
| Function | function:The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.,pathway:Protein modification; protein sumoylation.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the ubiquitin-activating E1 family.,subunit:Heterodimer of SAE1 and SAE2. The complex binds SUMO proteins via SAE2.,tissue specificity:Expression level increases during S phase and drops in G2 phase (at protein level)., |
| Subcellular location | Nucleus . |
| Expression | Expression level increases during S phase and drops in G2 phase (at protein level). |
Additional Images
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Western Blot analysis of various cells using SAE1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunofluorescence analysis of HUVEC cells, using AOS1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from 293 cells, using AOS1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES3404-50
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