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Antibody Product Overview
| ELK.No | ES3415 |
| Product name | SDHA rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC |
| Other name | SDHA; SDH2; SDHF; Succinate dehydrogenase [ubiquinone] flavoprotein subunit; mitochondrial; Flavoprotein subunit of complex II; Fp |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 6389 |
| Human Swiss-Prot | P31040 |
| Source | Rabbit |
| Isotype | IgG |
| Target | SDHA |
| Fields | >>Citrate cycle (TCA cycle);>>Oxidative phosphorylation;>>Metabolic pathways;>>Carbon metabolism;>>Thermogenesis;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Chemical carcinogenesis - reactive oxygen species;>>Diabetic cardiomyopathy |
| Gene name | SDHA |
| Protein name | Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 66945 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8K2B3 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 157074 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q920L2 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SDHA. AA range:551-600 |
| Specificity | SDHA Polyclonal Antibody detects endogenous levels of SDHA protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 70kD |
| Background | This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014], |
| Function | catalytic activity:Succinate + ubiquinone = fumarate + ubiquinol.,cofactor:FAD.,disease:Defects in SDHA are a cause of complex II mitochondrial respiratory chain deficiency [MIM:252011]; also known as succinate CoQ reductase deficiency. Defects of oxidative phosphorylation give rise to heterogeneous clinical symptoms ranging from isolated organ dysfunction to multisystem disorder. A deficiency of complex II represents a rare cause of mitochondrial encephalomyopathy, leukodystrophy, late-onset optic atrophy and ataxia, myopathy with exercise intolerance, and isolated cardiomyopathy.,disease:Defects in SDHA are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.,function:Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electr |
| Subcellular location | Mitochondrion inner membrane ; Peripheral membrane protein ; Matrix side . |
| Expression | Adipocyte,Brain,Colon,Heart,Liver,Placenta, |
Additional Images
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Western Blot analysis of various cells using SDHA Polyclonal Antibody diluted at 1:2000
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Western Blot analysis of HepG2 cells using SDHA Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysate from HeLa cells, using SDHA antibody.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES3415-100
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