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Antibody Product Overview
| ELK.No | ES3485 |
| Product name | Spindlin-1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | SPIN1; OCR; SPIN; Spindlin-1; Ovarian cancer-related protein |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 10927 |
| Human Swiss-Prot | Q9Y657 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Spindlin-1 |
| Fields | |
| Gene name | SPIN1 |
| Protein name | Spindlin-1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 20729 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q61142 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 1.00364e+008 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q4V8J7 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SPIN1. AA range:111-160 |
| Specificity | Spindlin-1 Polyclonal Antibody detects endogenous levels of Spindlin-1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 30kD |
| Background | function:May play a role in cell-cycle regulation during the transition from gamete to embryo.,PTM:Phosphorylated during oocyte meiotic maturation.,sequence caution:Translated as Glu.,similarity:Belongs to the SPIN/STSY family., |
| Function | function:May play a role in cell-cycle regulation during the transition from gamete to embryo.,PTM:Phosphorylated during oocyte meiotic maturation.,sequence caution:Translated as Glu.,similarity:Belongs to the SPIN/STSY family., |
| Subcellular location | Nucleus . Nucleus, nucleolus . |
| Expression | Highly expressed in ovarian cancer tissues. |
Additional Images
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Immunofluorescence analysis of Hela cell. 1,Spindlin-1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Bcl-2 Monoclonal Antibody(6B5)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
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Western Blot analysis of various cells using Spindlin-1 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western Blot analysis of HeLa cells using Spindlin-1 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
: AO-06-ES3485-100
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