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Antibody Product Overview
| ELK.No | ES3505 |
| Product name | ST5 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA |
| Other name | ST5; DENND2B; HTS1; Suppression of tumorigenicity 5 protein; DENN domain-containing protein 2B; HeLa tumor suppression 1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 6764 |
| Human Swiss-Prot | P78524 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ST5 |
| Fields | |
| Gene name | ST5 |
| Protein name | Suppression of tumorigenicity 5 protein |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 76954 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q924W7 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ST5. AA range:411-460 |
| Specificity | ST5 Polyclonal Antibody detects endogenous levels of ST5 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 126kD |
| Background | This gene was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein encoded by this gene contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells. Three alternatively spliced transcript variants of this gene encoding distinct isoforms are identified. [provided by RefSeq, Jul 2008], |
| Function | function:May be involved in cytoskeletal organization and tumorgenicity. Isoform 1 seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Isoform 3 may block ERK2 activation stimulated by ABL1. Isoform 3 may alter cell morphology and cell growth.,similarity:Contains 1 dDENN domain.,similarity:Contains 1 DENN domain.,similarity:Contains 1 uDENN domain.,subunit:Isoform 1 interacts with the SH3 domain of ABL1.,tissue specificity:Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level)., |
| Subcellular location | [Isoform 1]: Cytoplasm, cell cortex . Cell membrane . Recycling endosome . Colocalizes with RAB13 and ITSN1 at cytoplasmic vesicles that are most likely recycling endosomes. Colocalizes with the cortical actin cytoskeleton. . |
| Expression | Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level). |
Additional Images
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Western Blot analysis of various cells using ST5 Polyclonal Antibody
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Western Blot analysis of COLO205 cells using ST5 Polyclonal Antibody
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Western blot analysis of lysates from COLO205 cells, using ST5 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from COLO205 cells using ST5 antibody.
: AO-06-ES3505-100
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