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Antibody Product Overview
| ELK.No | ES3583 |
| Product name | Tetraspanin-8 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | TSPAN8; TM4SF3; Tetraspanin-8; Tspan-8; Transmembrane 4 superfamily member 3; Tumor-associated antigen CO-029 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 7103 |
| Human Swiss-Prot | P19075 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Tetraspanin-8 |
| Fields | |
| Gene name | TSPAN8 |
| Protein name | Tetraspanin-8 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q8R3G9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TSPAN8. AA range:96-145 |
| Specificity | Tetraspanin-8 Polyclonal Antibody detects endogenous levels of Tetraspanin-8 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 26kD |
| Background | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008], |
| Function | similarity:Belongs to the tetraspanin (TM4SF) family.,tissue specificity:Gastric, colon, rectal, and pancreatic carcinomas., |
| Subcellular location | Membrane; Multi-pass membrane protein. |
| Expression | Gastric, colon, rectal, and pancreatic carcinomas. |
Additional Images
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Western Blot analysis of various cells using Tetraspanin-8 Polyclonal Antibody diluted at 1:1000
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Immunofluorescence analysis of HeLa cells, using TSPAN8 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from 293 and COLO cells, using TSPAN8 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES3583-50
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