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Antibody Product Overview
| ELK.No | ES3649 |
| Product name | Trypsin-3 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | PRSS3; PRSS4; TRY3; TRY4; Trypsin-3; Brain trypsinogen; Mesotrypsinogen; Serine protease 3; Serine protease 4; Trypsin III; Trypsin IV |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 5646 |
| Human Swiss-Prot | P35030 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Trypsin-3 |
| Fields | >>Neuroactive ligand-receptor interaction;>>Pancreatic secretion;>>Protein digestion and absorption;>>Influenza A |
| Gene name | PRSS3 |
| Protein name | Trypsin-3 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Trypsin-3. AA range:117-166 |
| Specificity | Trypsin-3 Polyclonal Antibody detects endogenous levels of Trypsin-3 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 32kD |
| Background | This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene is localized to the locus of T cell receptor beta variable orphans on chromosome 9. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2010], |
| Function | catalytic activity:Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.,cofactor:Binds 1 calcium ion per subunit.,similarity:Belongs to the peptidase S1 family.,similarity:Contains 1 peptidase S1 domain.,tissue specificity:Pancreas and brain., |
| Subcellular location | Secreted . |
| Expression | Detected in pancreas and pancreatic fluid (at protein level) (PubMed:6698368). Expressed in pancreas and brain (PubMed:8294000). Detected in ileum (PubMed:12021776). |
Additional Images
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Western Blot analysis of various cells using Trypsin-3 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Western blot analysis of lysate from A549 cells, using Trypsin-3 antibody.
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: AO-06-ES3649-100
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