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Antibody Product Overview
| ELK.No | ES3732 |
| Product name | ZIS rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | ZRANB2; ZIS; ZNF265; Zinc finger Ran-binding domain-containing protein 2; Zinc finger protein 265; Zinc finger; splicing |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 9406 |
| Human Swiss-Prot | O95218 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ZIS |
| Fields | |
| Gene name | ZRANB2 |
| Protein name | Zinc finger Ran-binding domain-containing protein 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 53861 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9R020 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 58821 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | O35986 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ZNF265. AA range:106-155 |
| Specificity | ZIS Polyclonal Antibody detects endogenous levels of ZIS protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 34kD |
| Background | domain:The RanBP2-type zinc fingers mediate binding to RNA.,function:Splice factor required for alternative splicing of TRA2B/SFRS10 transcripts. May interfere with constitutive 5'-splice site selection.,PTM:Isoform 2 is phosphorylated on Ser-310 upon DNA damage, probably by ATM or ATR.,sequence caution:Intron retention.,similarity:Belongs to the ZRANB2 family.,similarity:Contains 2 RanBP2-type zinc fingers.,subunit:Interacts with the C-terminal half of SNRNP70, the Arg/Ser-rich domain of XE7 as well as with U2AF1 and CLK1., |
| Function | domain:The RanBP2-type zinc fingers mediate binding to RNA.,function:Splice factor required for alternative splicing of TRA2B/SFRS10 transcripts. May interfere with constitutive 5'-splice site selection.,PTM:Isoform 2 is phosphorylated on Ser-310 upon DNA damage, probably by ATM or ATR.,sequence caution:Intron retention.,similarity:Belongs to the ZRANB2 family.,similarity:Contains 2 RanBP2-type zinc fingers.,subunit:Interacts with the C-terminal half of SNRNP70, the Arg/Ser-rich domain of XE7 as well as with U2AF1 and CLK1., |
| Subcellular location | Nucleus . |
| Expression | Brain,Epithelium,Fetal brain,Fetal kidney,Liver,Skin,Testis,Uterus, |
Additional Images
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Western Blot analysis of various cells using ZIS Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunofluorescence analysis of HUVEC cells, using ZNF265 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HeLa cells, using ZNF265 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES3732-50
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