Français
English
Antibody Product Overview
| ELK.No | ES3748 |
| Product name | β-1,3-Gal-T4 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | B3GALT4; GALT4; Beta-1; 3-galactosyltransferase 4; Beta-1,3-GalTase 4; Beta3Gal-T4; Beta3GalT4; GalT4; b3Gal-T4; Gal-T2; Ganglioside galactosyltransferase; UDP-galactose:beta-N-acetyl-galactosamine-beta-1,3-galactosyltransferase |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 8705 |
| Human Swiss-Prot | O96024 |
| Source | Rabbit |
| Isotype | IgG |
| Target | β-1,3-Gal-T4 |
| Fields | >>Glycosphingolipid biosynthesis - ganglio series;>>Metabolic pathways |
| Gene name | B3GALT4 |
| Protein name | Beta-1,3-galactosyltransferase 4 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q9Z0F0 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human B3GALT4. AA range:181-230 |
| Specificity | β-1,3-Gal-T4 Polyclonal Antibody detects endogenous levels of β-1,3-Gal-T4 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 42kD |
| Background | This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3 |
| Function | catalytic activity:UDP-galactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-(1->4)-beta-D-glucosyl-N-acylsphingosine = UDP + D-galactosyl-(1->3)-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine.,function:Involved in GM1/GD1B/GA1 ganglioside biosynthesis.,online information:Beta-1,3-galactosyltransferase 4,online information:GlycoGene database,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyltransferase 31 family.,tissue specificity:Highly expressed in heart, skeletal muscle and pancreas and, to a lesser extent, in brain, placenta, kidney, liver and lung., |
| Subcellular location | Golgi apparatus membrane ; Single-pass type II membrane protein . |
| Expression | Highly expressed in heart, skeletal muscle and pancreas and, to a lesser extent, in brain, placenta, kidney, liver and lung. |
Additional Images
No image
Western Blot analysis of various cells using β-1,3-Gal-T4 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
No image
Western blot analysis of lysates from Jurkat cells, using B3GALT4 Antibody. The lane on the right is blocked with the synthesized peptide.
No image
Western blot analysis of the lysates from 293 cells using B3GALT4 antibody.
No image
: AO-06-ES3748-50
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package