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Antibody Product Overview
| ELK.No | ES3756 |
| Product name | V-ATPase D1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | ATP6V0D1; ATP6D; VPATPD; V-type proton ATPase subunit d 1; V-ATPase subunit d 1; 32 kDa accessory protein; V-ATPase 40 kDa accessory protein; V-ATPase AC39 subunit; p39; Vacuolar proton pump subunit d 1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 9114 |
| Human Swiss-Prot | P61421 |
| Source | Rabbit |
| Isotype | IgG |
| Target | V-ATPase D1 |
| Fields | >>Oxidative phosphorylation;>>Metabolic pathways;>>Lysosome;>>Phagosome;>>Synaptic vesicle cycle;>>Collecting duct acid secretion;>>Vibrio cholerae infection;>>Epithelial cell signaling in Helicobacter pylori infection;>>Tuberculosis;>>Human papillomavirus infection;>>Viral carcinogenesis;>>Rheumatoid arthritis |
| Gene name | ATP6V0D1 |
| Protein name | V-type proton ATPase subunit d 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 11972 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P51863 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human V-ATPase D1. AA range:221-270 |
| Specificity | V-ATPase D1 Polyclonal Antibody detects endogenous levels of V-ATPase D1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 40kD |
| Background | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously. [pro |
| Function | function:Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis.,similarity:Belongs to the V-ATPase V0D/AC39 subunit family.,subunit:V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d).,tissue specificity:Ubiquitous., |
| Subcellular location | Membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome membrane ; Peripheral membrane protein . Cytoplasmic vesicle, clathrin-coated vesicle membrane ; Peripheral membrane protein . Localizes to centrosome and the base of the cilium. . |
| Expression | Ubiquitous. |
Additional Images
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Western Blot analysis of extracts from NIH-3T3, K562 cells, using V-ATPase D1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Western blot analysis of lysates from HeLa cells, using V-ATPase D1 antibody.
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: AO-06-ES3756-50
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