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Antibody Product Overview
| ELK.No | ES3947 |
| Product name | MCM3 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | MCM3; DNA replication licensing factor MCM3; DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 4172 |
| Human Swiss-Prot | P25205 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MCM3 |
| Fields | >>DNA replication;>>Cell cycle |
| Gene name | MCM3 |
| Protein name | DNA replication licensing factor MCM3 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | P25206 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human MCM3. AA range:641-690 |
| Specificity | MCM3 Polyclonal Antibody detects endogenous levels of MCM3 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 89kD |
| Background | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012], |
| Function | function:Acts as a factor that allows the DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation.,miscellaneous:Associated with the replication-specific DNA polymerase alpha.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain., |
| Subcellular location | Nucleus . Chromosome . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. . |
| Expression | Cervix,Coronary artery,Epithelium,Lymph,Osteosarcoma,Pancre |
Additional Images
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Western Blot analysis of K562 cells using MCM3 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100
: AO-06-ES3947-100
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