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Antibody Product Overview
| ELK.No | ES3978 |
| Product name | Integrin α4 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | ITGA4; CD49D; Integrin alpha-4; CD49 antigen-like family member D; Integrin alpha-IV; VLA-4 subunit alpha; CD49d |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3676 |
| Human Swiss-Prot | P13612 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Integrin α4 |
| Fields | >>PI3K-Akt signaling pathway;>>Focal adhesion;>>ECM-receptor interaction;>>Cell adhesion molecules;>>Hematopoietic cell lineage;>>Leukocyte transendothelial migration;>>Intestinal immune network for IgA production;>>Regulation of actin cytoskeleton;>>Yersinia infection;>>Leishmaniasis;>>Human papillomavirus infection;>>Hypertrophic cardiomyopathy;>>Arrhythmogenic right ventricular cardiomyopathy;>>Dilated cardiomyopathy |
| Gene name | ITGA4 |
| Protein name | Integrin alpha-4 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 16401 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q00651 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human ITGA4. AA range:571-620 |
| Specificity | Integrin α4 Polyclonal Antibody detects endogenous levels of Integrin α4 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 120kD |
| Background | integrin subunit alpha 4(ITGA4) Homo sapiens The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015], |
| Function | domain:The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.,function:Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.,PTM:Phosphorylation on Ser-1027 inhibits PXN binding.,similarity:Belongs to the integrin alpha chain family.,similarity:Contains 7 FG-GAP repeats.,subunit:Heterodimer of an alpha and a beta subunit |
| Subcellular location | Membrane; Single-pass type I membrane protein. |
| Expression | Lymph,Peripheral blood,Prostate,Spleen,Testis, |
Additional Images
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Western Blot analysis of K562 cells using Integrin α4 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human-lymph, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded human-lymph, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded human-lymph, antibody was diluted at 1:100
: AO-06-ES3978-50
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