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Antibody Product Overview
| ELK.No | ES4066 |
| Product name | MyoD rabbit pAb |
| Reactivity | Human;Mouse;Rat;Chicken(testedbyourcustomer） |
| Applications | WB;IHC |
| Other name | MYOD1; BHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 4654 |
| Human Swiss-Prot | P15172 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MyoD |
| Fields | >>Spinocerebellar ataxia |
| Gene name | MYOD1 |
| Protein name | Myoblast determination protein 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 17927 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P10085 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 337868 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q02346 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MyoD around the non-acetylation site of Lys99/102. AA range:61-110 |
| Specificity | MyoD Polyclonal Antibody detects endogenous levels of MyoD protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 35kD |
| Background | This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008], |
| Function | function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.,online information:MyoD entry,PTM:Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.,PTM:Ubiquitinated on the N-terminus; which is required for proteasomal degradation.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1., |
| Subcellular location | Nucleus. |
| Expression | Muscle,Skeletal muscle, |
Additional Images
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Western Blot analysis of Jurkat cells using MyoD Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of extracts from Jurkat cells, using MyoD (Ab-99/102) Antibody.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES4066-50
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