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Antibody Product Overview
| ELK.No | ES4101 |
| Product name | MCM7 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | MCM7; CDC47; MCM2; DNA replication licensing factor MCM7; CDC47 homolog; P1.1-MCM3 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 4176 |
| Human Swiss-Prot | P33993 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MCM7 |
| Fields | >>DNA replication;>>Cell cycle |
| Gene name | MCM7 |
| Protein name | DNA replication licensing factor MCM7 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 17220 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q61881 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human MCM7. AA range:491-540 |
| Specificity | MCM7 Polyclonal Antibody detects endogenous levels of MCM7 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 81kD |
| Background | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008], |
| Function | function:Acts as a factor that allows the DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation. Required for S-phase checkpoint activation upon UV-induced damage.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain.,subunit:Interacts with the ATR-ATRIP complex and with RAD17. Interacts with TIPIN., |
| Subcellular location | Nucleus . Chromosome . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. . |
| Expression | Brain,Cervix,Chronic myeloid leukemia cell,Lung, |
Additional Images
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Western Blot analysis of 293 cells using MCM7 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
: AO-06-ES4101-50
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