Français
English
Antibody Product Overview
| ELK.No | ES4116 |
| Product name | 17β-HSD4 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | HSD17B4; EDH17B4; Peroxisomal multifunctional enzyme type 2; MFE-2; 17-beta-hydroxysteroid dehydrogenase 4; 17-beta-HSD 4; D-bifunctional protein; DBP; Multifunctional protein 2; MPF-2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3295 |
| Human Swiss-Prot | P51659 |
| Source | Rabbit |
| Isotype | IgG |
| Target | 17β-HSD4 |
| Fields | >>Primary bile acid biosynthesis;>>Biosynthesis of unsaturated fatty acids;>>Metabolic pathways;>>Fatty acid metabolism;>>Peroxisome |
| Gene name | HSD17B4 |
| Protein name | Peroxisomal multifunctional enzyme type 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 15488 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P51660 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 79244 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P97852 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from the N-terminal region of human HSD17B4. AA range:41-90 |
| Specificity | 17β-HSD4 Polyclonal Antibody detects endogenous levels of 17β-HSD4 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 80kD |
| Background | hydroxysteroid 17-beta dehydrogenase 4(HSD17B4) Homo sapiens The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014], |
| Function | catalytic activity:(24R,25R)-3-alpha,7-alpha,12-alpha,24-tetrahydroxy-5-beta-cholestanoyl-CoA = (24E)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA + H(2)O.,catalytic activity:(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.,disease:Defects in HSD17B4 are a cause of D-bifunctional protein deficiency (DBPD) [MIM:261515]. DBPD is a disorder of peroxisomal fatty acid beta-oxidation.,function:Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids.,pathway:Lipid metabolism; fatty acid beta-oxidation.,similarity:Belongs to the short-chain dehydrogenases/reductases (SDR) family.,similarity:Contains 1 SCP2 domain.,tissue specificity:Present in many tissues with highest concentrations in liver, heart, prostate and testis., |
| Subcellular location | Peroxisome . |
| Expression | Present in many tissues with highest concentrations in liver, heart, prostate and testis. |
Additional Images
No image
Western Blot analysis of HuvEc, K562 cells using 17β-HSD4 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
No image
Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100
No image
Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
No image
Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
: AO-06-ES4116-50
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package