Français
English
Antibody Product Overview
| ELK.No | ES4146 |
| Product name | IDH1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | IDH1; PICD; Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 3417 |
| Human Swiss-Prot | O75874 |
| Source | Rabbit |
| Isotype | IgG |
| Target | IDH1 |
| Fields | >>Citrate cycle (TCA cycle);>>Glutathione metabolism;>>Metabolic pathways;>>Carbon metabolism;>>2-Oxocarboxylic acid metabolism;>>Biosynthesis of amino acids;>>Peroxisome;>>Central carbon metabolism in cancer |
| Gene name | IDH1 |
| Protein name | Isocitrate dehydrogenase [NADP] cytoplasmic |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 15926 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | O88844 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24479 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P41562 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from the N-terminal region of human IDH1. |
| Specificity | IDH1 Polyclonal Antibody detects endogenous levels of IDH1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 46kD |
| Background | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to |
| Function | catalytic activity:Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.,catalytic activity:Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.,cofactor:Binds 1 magnesium or manganese ion per subunit.,disease:Defects in IDH1 are a cause of glioblastoma multiforme (GBM) [MIM:137800]; also called familial glioma of brain. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas.,miscellaneous:Cancer mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas.,online information:Isocitrate dehydrogenase entry,similarity:Belongs to the isocitrate and isopropylmalate dehydrogenases family.,subunit:Homodimer., |
| Subcellular location | Cytoplasm, cytosol . Peroxisome . |
| Expression | Brain,Cajal-Retzius cell,Fetal brain cortex,Human endometri |
Additional Images
No image
Western Blot analysis of 293, HeLa cells using IDH1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
No image
Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
No image
Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
No image
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100
: AO-06-ES4146-100
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package