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Antibody Product Overview
| ELK.No | ES4374 |
| Product name | Lefty rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC |
| Other name | LEFTY1; LEFTB; LEFTYB; Left-right determination factor 1; Left-right determination factor B; Protein lefty-1; Protein lefty-B; LEFTY2; EBAF; LEFTA; LEFTYA; TGFB4; PSEC0024; Left-right determination factor 2; Endometrial bleeding-associated factor; Left-right determination factor A; Protein lefty-2; Protein lefty-A; Transforming growth factor beta-4; TGF-beta-4 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 10637/7044 |
| Human Swiss-Prot | O75610/O00292 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Lefty |
| Fields | >>TGF-beta signaling pathway;>>Signaling pathways regulating pluripotency of stem cells |
| Gene name | LEFTY1/LEFTY2 |
| Protein name | Left-right determination factor 1/Left-right determination factor 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 13590/320202 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human LEFTY1/LEFTY2. AA range:301-350 |
| Specificity | Lefty Polyclonal Antibody detects endogenous levels of Lefty protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 40kD |
| Background | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This gene is closely linked to both a related family member and a related pseudogene. [provided by RefSeq, Aug 2016], |
| Function | function:Required for left-right axis determination as a regulator of LEFTY2 and NODAL.,PTM:The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.,similarity:Belongs to the TGF-beta family., |
| Subcellular location | Secreted. |
| Expression | Colon,Pancreas,Spleen,Teratocarcinoma, |
Additional Images
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Western Blot analysis of HepG2-UV cells using Lefty Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Western Blot analysis of HEPG2-UV cells using Lefty Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES4374-50
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