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Antibody Product Overview
| ELK.No | ES4382 |
| Product name | IRF-1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | IRF1; Interferon regulatory factor 1; IRF-1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3659 |
| Human Swiss-Prot | P10914 |
| Source | Rabbit |
| Isotype | IgG |
| Target | IRF-1 |
| Fields | >>C-type lectin receptor signaling pathway;>>TNF signaling pathway;>>Prolactin signaling pathway;>>Pertussis;>>Human papillomavirus infection |
| Gene name | IRF1 |
| Protein name | Interferon regulatory factor 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 16362 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P15314 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24508 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P23570 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human IRF1. AA range:91-140 |
| Specificity | IRF-1 Polyclonal Antibody detects endogenous levels of IRF-1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 36kD |
| Background | IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion. [provided by RefSeq, Jul 2008], |
| Function | disease:Deletion or rearrangement of IRF1 are a cause of preleukemic myelodysplastic syndrome (MDS) and of acute myelogenous leukemia (AML).,function:Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and activates those genes. Acts as a tumor suppressor.,induction:By viruses and IFN.,PTM:Sumoylation represses the transcriptional activity and displays enhanced resistance to protein degradation. Inactivates the tumor suppressor activity. Elevated levels in tumor cells. Major site is Lys-275. Sumoylation is enhanced by PIAS3 (By similarity). Desumoylated by SENP1 in tumor cells and appears to compete with ubiquitination on C-terminal sites.,PTM:Ubiquitinated. Appears to compete with sumoylation on C-terminal sites.,similarity:Belongs to the IRF family.,similarity:Contains 1 tryptophan pentad repea |
| Subcellular location | Nucleus . Cytoplasm . MYD88-associated IRF1 migrates into the nucleus more efficiently than non-MYD88-associated IRF1. . |
| Expression | Pancreas,Placenta,Spleen, |
Additional Images
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Western Blot analysis of PC12 cells using IRF-1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES4382-50
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