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C/EBP α (phospho Thr226) rabbit pAb
AO-06-ES4487-50
Antibody Product Overview
| ELK.No | ES4487 |
| Product name | C/EBP α (phospho Thr226) rabbit pAb |
| Reactivity | Human;Mouse;Rat;Monkey |
| Applications | WB;ELISA |
| Other name | CEBPA; CCAAT/enhancer-binding protein alpha; C/EBP alpha |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 1050 |
| Human Swiss-Prot | P49715 |
| Source | Rabbit |
| Isotype | IgG |
| Target | C/EBP α |
| Fields | >>Non-alcoholic fatty liver disease;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Acute myeloid leukemia |
| Gene name | CEBPA |
| Protein name | CCAAT/enhancer-binding protein alpha |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 12606 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P53566 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24252 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P05554 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human C/EBP-alpha around the phosphorylation site of Thr226. AA range:192-241 |
| Specificity | Phospho-C/EBP α (T226) Polyclonal Antibody detects endogenous levels of C/EBP α protein only when phosphorylated at T226. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 42,also have 30kd isform |
| Background | This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013], |
| Function | function:C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. C/EBP subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with UBN1. Interacts with HBV protein X., |
| Subcellular location | Nucleus .; [Isoform 4]: Nucleus, nucleolus . |
| Expression | Liver,Pancreas,Umbilical cord,White Matter pool- 5 brain tissues- f |
Additional Images
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using C/EBP-alpha (Phospho-Thr226) Antibody
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Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using C/EBP-alpha (Phospho-Thr226) Antibody. The lane on the right is blocked with the phospho peptide.
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: AO-06-ES4487-50
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