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C/EBP ε (phospho Thr74) rabbit pAb
AO-06-ES4500-50
Antibody Product Overview
| ELK.No | ES4500 |
| Product name | C/EBP ε (phospho Thr74) rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | CEBPE; CCAAT/enhancer-binding protein epsilon; C/EBP epsilon |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 1053 |
| Human Swiss-Prot | Q15744 |
| Source | Rabbit |
| Isotype | IgG |
| Target | C/EBP ε |
| Fields | >>Transcriptional misregulation in cancer;>>Acute myeloid leukemia |
| Gene name | CEBPE |
| Protein name | CCAAT/enhancer-binding protein epsilon |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q6PZD9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human C/EBP-epsilon around the phosphorylation site of Thr74. AA range:40-89 |
| Specificity | Phospho-C/EBP ε (T74) Polyclonal Antibody detects endogenous levels of C/EBP ε protein only when phosphorylated at T74. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 34kD |
| Background | The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008], |
| Function | function:C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.,online information:CEBPE mutation db,PTM:Phosphorylated.,similarity:Belongs to the bZIP family. C/EBP subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a dimer and can form stable heterodimers with C/EBP delta.,tissue specificity:Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines., |
| Subcellular location | Nucleus . |
| Expression | Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines. |
Additional Images
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using C/EBP-epsilon (Phospho-Thr74) Antibody
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Immunofluorescence analysis of HeLa cells, using C/EBP-epsilon (Phospho-Thr74) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain, using C/EBP-epsilon (Phospho-Thr74) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HUVEC cells treated with UV 15', using C/EBP-epsilon (Phospho-Thr74) Antibody. The lane on the right is blocked with the phospho peptide.
: AO-06-ES4500-50
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