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Antibody Product Overview
| ELK.No | ES4503 |
| Product name | C/EBP γ rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | CEBPG; CCAAT/enhancer-binding protein gamma; C/EBP gamma |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 1054 |
| Human Swiss-Prot | P53567 |
| Source | Rabbit |
| Isotype | IgG |
| Target | C/EBP γ |
| Fields | >>Tuberculosis |
| Gene name | CEBPG |
| Protein name | CCAAT/enhancer-binding protein gamma |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 12611 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P53568 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25301 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P26801 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CEBPG. AA range:31-80 |
| Specificity | C/EBP γ Polyclonal Antibody detects endogenous levels of C/EBP γ protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 22kD |
| Background | The C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein: a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. The C/EBP family consist of several related proteins, C/EBP alpha, C/EBP beta, C/EBP gamma, and C/EBP delta, that form homodimers and that form heterodimers with each other. CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Nov 2011], |
| Function | function:Transcription factor that binds to the enhancer element PRE-I (positive regulatory element-I) of the IL-4 gene. Might change the DNA-binding specificity of other transcription factors and recruit them to unusual DNA sites.,similarity:Belongs to the bZIP family. C/EBP subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha and beta and with Fos protein., |
| Subcellular location | Nucleus . |
| Expression | Placenta,T-cell,Trachea, |
Additional Images
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Western Blot analysis of RAW264.7 cells using C/EBP γ Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CEBPG Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from RAW264.7 cells, using CEBPG Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES4503-100
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