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Antibody Product Overview
| ELK.No | ES4504 |
| Product name | PHAPI2 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | ANP32B; APRIL; PHAPI2; Acidic leucine-rich nuclear phosphoprotein 32 family member B; Acidic protein rich in leucines; Putative HLA-DR-associated protein I-2; PHAPI2; Silver-stainable protein SSP29 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 10541 |
| Human Swiss-Prot | Q92688 |
| Source | Rabbit |
| Isotype | IgG |
| Target | PHAPI2 |
| Fields | |
| Gene name | ANP32B |
| Protein name | Acidic leucine-rich nuclear phosphoprotein 32 family member B |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 67628 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9EST5 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 170724 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q9EST6 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ANP32B. AA range:1-50 |
| Specificity | PHAPI2 Polyclonal Antibody detects endogenous levels of PHAPI2 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 29kD |
| Background | function:Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity.,similarity:Belongs to the ANP32 family.,similarity:Contains 4 LRR (leucine-rich) repeats.,subcellular location:Accumulates in the nuclei at the S phase.,tissue specificity:Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta., |
| Function | function:Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity.,similarity:Belongs to the ANP32 family.,similarity:Contains 4 LRR (leucine-rich) repeats.,subcellular location:Accumulates in the nuclei at the S phase.,tissue specificity:Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta., |
| Subcellular location | [Isoform 1]: Nucleus . Cytoplasm . Accumulates in the nuclei at the S phase. .; [Isoform 2]: Cytoplasm. Lacks a nuclear localization signal. |
| Expression | Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta. |
Additional Images
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Western Blot analysis of rat cells using PHAPI2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of lysates from rat brain cells, using ANP32B Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HeLa cells using ANP32B antibody.
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Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES4504-100
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