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Antibody Product Overview
| ELK.No | ES4528 |
| Product name | RGS14 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | RGS14; Regulator of G-protein signaling 14; RGS14 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 10636 |
| Human Swiss-Prot | O43566 |
| Source | Rabbit |
| Isotype | IgG |
| Target | RGS14 |
| Fields | >>Rap1 signaling pathway |
| Gene name | RGS14 |
| Protein name | Regulator of G-protein signaling 14 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 51791 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P97492 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 114705 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | O08773 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RGS14. AA range:125-174 |
| Specificity | RGS14 Polyclonal Antibody detects endogenous levels of RGS14 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 65kD |
| Background | This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008], |
| Function | function:Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form.,similarity:Contains 1 GoLoco domain.,similarity:Contains 1 RGS domain.,similarity:Contains 2 RBD (Ras-binding) domains., |
| Subcellular location | Nucleus . Nucleus, PML body . Cytoplasm . Membrane . Cell membrane . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, spindle pole . Cell projection, dendrite . Cell projection, dendritic spine . Cell junction, synapse, postsynaptic density . Associates with the perinuclear sheaths of microtubules (MTs) surrounding the pronuclei, prior to segregating to the anastral mitotic apparatus and subsequently the barrel-shaped cytoplasmic bridge between the nascent nuclei of the emerging 2-cell embryo. Localizes to a perinuclear compartment near the microtubule-organizing center (MTOC). Expressed in the nucleus during interphase and segregates to the centrosomes and astral MTs during mitosis. Relocalizes to the nucleus |
| Expression | Brain,Caudate nucleus,Colon, |
Additional Images
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Western Blot analysis of 3T3 cells using RGS14 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of the lysates from HUVECcells using RGS14 antibody.
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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES4528-50
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