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Antibody Product Overview
| ELK.No | ES4566 |
| Product name | MRP-S30 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC |
| Other name | MRPS30; PDCD9; BM-047; 28S ribosomal protein S30; mitochondrial; MRP-S30; S30mt; Programmed cell death protein 9 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 10884 |
| Human Swiss-Prot | Q9NP92 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MRP-S30 |
| Fields | |
| Gene name | MRPS30 |
| Protein name | 28S ribosomal protein S30 mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q9D0G0 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPS30. AA range:390-439 |
| Specificity | MRP-S30 Polyclonal Antibody detects endogenous levels of MRP-S30 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 55kD |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences i |
| Function | sequence caution:Sequencing errors.,similarity:Belongs to the ribosomal protein S30/L37 family.,subunit:Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.,tissue specificity:Heart, skeletal muscle, kidney and liver. Lower expression in placenta and peripheral blood leukocytes., |
| Subcellular location | Mitochondrion . |
| Expression | Heart, skeletal muscle, kidney and liver. Lower expression in placenta and peripheral blood leukocytes. |
Additional Images
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Western Blot analysis of LOVO cells using MRP-S30 Polyclonal Antibody
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Western blot analysis of lysates from LOVO cells, using MRPS30 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from Jurkat cells using MRPS30 antibody.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES4566-50
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