Français
English
Antibody Product Overview
| ELK.No | ES4574 |
| Product name | ACOT2 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | ACOT2; PTE2; PTE2A; Acyl-coenzyme A thioesterase 2; mitochondrial; Acyl-CoA thioesterase 2; Acyl-coenzyme A thioester hydrolase 2a; CTE-Ia; Long-chain acyl-CoA thioesterase 2; ZAP128 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 10965 |
| Human Swiss-Prot | P49753 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ACOT2 |
| Fields | >>Fatty acid elongation;>>Biosynthesis of unsaturated fatty acids;>>Metabolic pathways;>>Ovarian steroidogenesis |
| Gene name | ACOT2 |
| Protein name | Acyl-coenzyme A thioesterase 2 mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q9QYR9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ACOT2. AA range:171-220 |
| Specificity | ACOT2 Polyclonal Antibody detects endogenous levels of ACOT2 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 53kD |
| Background | This gene encodes a member of the acyl-CoA thioesterase protein family, and is one of four acyl-CoA hydrolase genes located in a cluster on chromosome 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012], |
| Function | catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,caution:Was originally (PubMed:10944470) thought to be peroxisomal but was later shown (PubMed:16940157) to be mitochondrial.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Displays high levels of activity on medium- and long chain acyl CoAs.,similarity:Belongs to the C/M/P thioester hydrolase family.,tissue specificity:Strongest expression in heart, liver, muscle and kidney. Weak in placenta and pancreas., |
| Subcellular location | Mitochondrion . |
| Expression | Strongest expression in heart, liver, muscle and kidney. Weak in placenta and pancreas. |
Additional Images
No image
Western Blot analysis of VEC cells using ACOT2 Polyclonal Antibody diluted at 1:500
No image
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
No image
Western blot analysis of lysates from Jurkat and HUVEC cells, using ACOT2 Antibody. The lane on the right is blocked with the synthesized peptide.
No image
Western blot analysis of the lysates from HepG2 cells using ACOT2 antibody.
: AO-06-ES4574-50
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package