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KIF2C (phospho Ser95) rabbit pAb
AO-06-ES4579-50
Antibody Product Overview
| ELK.No | ES4579 |
| Product name | KIF2C (phospho Ser95) rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC |
| Other name | KIF2C; KNSL6; Kinesin-like protein KIF2C; Kinesin-like protein 6; Mitotic centromere-associated kinesin; MCAK |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 11004 |
| Human Swiss-Prot | Q99661 |
| Source | Rabbit |
| Isotype | IgG |
| Target | KIF2C |
| Fields | |
| Gene name | KIF2C |
| Protein name | Kinesin-like protein KIF2C |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 73804 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q922S8 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human KIF2C around the phosphorylation site of Ser95. AA range:61-110 |
| Specificity | Phospho-KIF2C (S95) Polyclonal Antibody detects endogenous levels of KIF2C protein only when phosphorylated at S95. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 80kD |
| Background | kinesin family member 2C(KIF2C) Homo sapiens This gene encodes a kinesin-like protein that functions as a microtubule-dependent molecular motor. The encoded protein can depolymerize microtubules at the plus end, thereby promoting mitotic chromosome segregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014], |
| Function | function:Present throughout the cell cycle, associates with centromeres at early prophase, and remains associated with the centromere until after telophase.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the kinesin-like protein family.,similarity:Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.,similarity:Contains 1 kinesin-motor domain.,subunit:Interacts with CENPH.,tissue specificity:Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas., |
| Subcellular location | Cytoplasm, cytoskeleton . Nucleus . Chromosome, centromere . Chromosome, centromere, kinetochore . Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGO2. . |
| Expression | Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific. |
Additional Images
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using KIF2C (Phospho-Ser95) Antibody
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Immunofluorescence analysis of A549 cells, using KIF2C (Phospho-Ser95) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HeLa cells treated with TNF 10ng/ml 30', using KIF2C (Phospho-Ser95) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES4579-50
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