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Antibody Product Overview
| ELK.No | ES4624 |
| Product name | MAN1B1 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | MAN1B1; Endoplasmic reticulum mannosyl-oligosaccharide 1; 2-alpha-mannosidase; ER alpha-1,2-mannosidase; ER mannosidase 1; ERMan1; Man9GlcNAc2-specific-processing alpha-mannosidase; Mannosidase alpha class 1B member 1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 11253 |
| Human Swiss-Prot | Q9UKM7 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MAN1B1 |
| Fields | >>N-Glycan biosynthesis;>>Various types of N-glycan biosynthesis;>>Metabolic pathways;>>Protein processing in endoplasmic reticulum |
| Gene name | MAN1B1 |
| Protein name | Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | A2AJ15 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized peptide derived from MAN1B1 . at AA range: 100-180 |
| Specificity | MAN1B1 Polyclonal Antibody detects endogenous levels of MAN1B1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 80kD |
| Background | This gene encodes an enzyme belonging to the glycosyl hydrolase 47 family. This enzyme functions in N-glycan biosynthesis, and is a class I alpha-1,2-mannosidase that specifically converts Man9GlcNAc to Man8GlcNAc isomer B. It is required for N-glycan trimming to Man5-6GlcNAc2 in the endoplasmic-reticulum-associated degradation pathway. Mutations in this gene cause autosomal-recessive intellectual disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. [provided by RefSeq, Dec 2011], |
| Function | catalytic activity:Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man(9)(GlcNAc)(2).,cofactor:Calcium.,enzyme regulation:Inhibited by both 1-deoxymannojirimycin and kifunensine.,function:Involved in the maturation of Asn-linked oligosaccharides. Trim a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2). The only product is the Man(8)GlcNAc(2) isomer B, the form lacking the middle-arm terminal alpha 1,2-mannose. It may be involved in glycoprotein quality control since it is important to target misfolded glycoproteins for degradation.,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyl hydrolase 47 family.,tissue specificity:Widely expressed., |
| Subcellular location | Endoplasmic reticulum membrane ; Single-pass type II membrane protein . |
| Expression | Widely expressed. |
Additional Images
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Western Blot analysis of 3T3 cells using MAN1B1 Polyclonal Antibody
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Western blot analysis of MAN1B1 Antibody. The lane on the right is blocked with the MAN1B1 peptide.
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: AO-06-ES4624-50
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