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Antibody Product Overview
| ELK.No | ES4650 |
| Product name | Rhophilin rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA;IHC |
| Other name | RHPN1; KIAA1929; Rhophilin-1; GTP-Rho-binding protein 1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 114822 |
| Human Swiss-Prot | Q8TCX5 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Rhophilin |
| Fields | |
| Gene name | RHPN1 |
| Protein name | Rhophilin-1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q61085 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RHPN1. AA range:474-523 |
| Specificity | Rhophilin Polyclonal Antibody detects endogenous levels of Rhophilin protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 100kD |
| Background | domain:The PDZ domain mediates interaction with ROPN1.,function:Has no enzymatic activity. May serve as a target for Rho, and interact with some cytoskeletal component upon Rho binding or relay a Rho signal to other molecules.,similarity:Belongs to the RHPN family.,similarity:Contains 1 BRO1 domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 REM (Hr1) repeat.,subunit:Binds specifically to GTP-Rho. Interacts with ROPN1., |
| Function | domain:The PDZ domain mediates interaction with ROPN1.,function:Has no enzymatic activity. May serve as a target for Rho, and interact with some cytoskeletal component upon Rho binding or relay a Rho signal to other molecules.,similarity:Belongs to the RHPN family.,similarity:Contains 1 BRO1 domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 REM (Hr1) repeat.,subunit:Binds specifically to GTP-Rho. Interacts with ROPN1., |
| Subcellular location | cytosol, |
| Expression | Blood,Brain, |
Additional Images
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Western Blot analysis of A549 cells using Rhophilin Polyclonal Antibody
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Western blot analysis of the lysates from HepG2 cells using RHPN1 antibody.
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES4650-50
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