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Antibody Product Overview
| ELK.No | ES4673 |
| Product name | ADD1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | IHC;IF;ELISA |
| Other name | ADD1; ADDA; Alpha-adducin; Erythrocyte adducin subunit alpha |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 118 |
| Human Swiss-Prot | P35611 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ADD1 |
| Fields | |
| Gene name | ADD1 |
| Protein name | Alpha-adducin |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 11518 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9QYC0 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24170 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q63028 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ADD1. AA range:411-460 |
| Specificity | ADD1 Polyclonal Antibody detects endogenous levels of ADD1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 81kD |
| Observed band (KD) | |
| Background | adducin 1(ADD1) Homo sapiens Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008], |
| Function | alternative products:Additional isoforms seem to exist,domain:Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.,function:Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.,PTM:The N-terminus is blocked.,similarity:Belongs to the aldolase class II family. Adducin subfamily.,subunit:Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit. Binds ROCK1.,tissue specificity:Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit., |
| Subcellular location | Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. |
| Expression | Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit. |
Additional Images
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Immunofluorescence analysis of HeLa cells, using ADD1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ADD1 Antibody. The picture on the right is blocked with the synthesized peptide.
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: AO-06-ES4673-100
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