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Antibody Product Overview
| ELK.No | ES4965 |
| Product name | DAPK1 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | DAPK1; DAPK; Death-associated protein kinase 1; DAP kinase 1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 1612 |
| Human Swiss-Prot | P53355 |
| Source | Rabbit |
| Isotype | IgG |
| Target | DAPK1 |
| Fields | >>Autophagy - animal;>>Pathways in cancer;>>Bladder cancer |
| Gene name | DAPK1 |
| Protein name | Death-associated protein kinase 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 69635 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q80YE7 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DAPK1. AA range:274-323 |
| Specificity | DAPK1 Polyclonal Antibody detects endogenous levels of DAPK1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 160kD |
| Background | Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013], |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-308.,function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,induction:Up-regulated following treatment with interferon-gamma.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 10 ANK repeats.,subcellular location:Colocalizes with the actin filament system., |
| Subcellular location | [Isoform 1]: Cytoplasm. Cytoplasm, cytoskeleton. Colocalizes with MAP1B in the microtubules and cortical actin fibers.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton. |
| Expression | Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas. |
Additional Images
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Western Blot analysis of MOUSE-BRAIN cells using DAPK1 Polyclonal Antibody diluted at 1:1000
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Immunofluorescence analysis of COS7 cells, using DAPK1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using DAPK1 Antibody. The picture on the right is blocked with the synthesized peptide.
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: AO-06-ES4965-100
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