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EDG-1 (phospho Thr236) rabbit pAb
AO-06-ES5040-100
Antibody Product Overview
| ELK.No | ES5040 |
| Product name | EDG-1 (phospho Thr236) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IF;ELISA |
| Other name | S1PR1; CHEDG1; EDG1; Sphingosine 1-phosphate receptor 1; S1P receptor 1; S1P1; Endothelial differentiation G-protein coupled receptor 1; Sphingosine 1-phosphate receptor Edg-1; S1P receptor Edg-1; CD antigen CD363 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 1901 |
| Human Swiss-Prot | P21453 |
| Source | Rabbit |
| Isotype | IgG |
| Target | EDG-1 |
| Fields | >>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Neuroactive ligand-receptor interaction |
| Gene name | S1PR1 |
| Protein name | Sphingosine 1-phosphate receptor 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 13609 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | O08530 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 29733 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P48303 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human S1P Receptor EDG1 around the phosphorylation site of Thr236. AA range:206-255 |
| Specificity | Phospho-EDG-1 (T236) Polyclonal Antibody detects endogenous levels of EDG-1 protein only when phosphorylated at T236. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 42kD |
| Background | The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016], |
| Function | function:Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.,induction:By the tumor promoter phorbol 12-myristate 13-acetate (PME) in the presence of cycloheximide.,PTM:S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin., |
| Subcellular location | Cell membrane ; Multi-pass membrane protein. Endosome. Membrane raft. Recruited to caveolin-enriched plasma membrane microdomains in response to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine. Ligand binding leads to receptor internalization. |
| Expression | Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. |
Additional Images
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Western blot analysis of HEPG2-UV MOUSE-BRAIN using p-EDG-1 (T236) antibody. Antibody was diluted at 1:500
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using S1P Receptor EDG1 (Phospho-Thr236) Antibody
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Immunofluorescence analysis of COS7 cells, using S1P Receptor EDG1 (Phospho-Thr236) Antibody. The picture on the right is blocked with the phospho peptide.
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: AO-06-ES5040-100
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