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EF-2 (phospho Thr56) rabbit pAb
AO-06-ES5044-50
Antibody Product Overview
| ELK.No | ES5044 |
| Product name | EF-2 (phospho Thr56) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | EEF2; EF2; Elongation factor 2; EF-2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 1938 |
| Human Swiss-Prot | P13639 |
| Source | Rabbit |
| Isotype | IgG |
| Target | eEF2 |
| Fields | >>AMPK signaling pathway;>>Oxytocin signaling pathway |
| Gene name | EEF2 |
| Protein name | Elongation factor 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 13629 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P58252 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 29565 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P05197 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human eEF2 around the phosphorylation site of Thr56. AA range:31-80 |
| Specificity | Phospho-EF-2 (T56) Polyclonal Antibody detects endogenous levels of EF-2 protein only when phosphorylated at T56. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 100kD |
| Background | This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008], |
| Function | function:This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome.,PTM:Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.,PTM:Phosphorylation by EF-2 kinase completely inactivates EF-2.,similarity:Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.,subunit:Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9., |
| Subcellular location | Cytoplasm . Nucleus . Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product. . |
| Expression | Brain,Cajal-Retzius cell,Epithelium,Hepatocyte,Ovary,Periph |
Additional Images
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Western Blot analysis of SKOV3 cells using Phospho-EF-2 (T56) Polyclonal Antibody diluted at 1:2000
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eEF2 (Phospho-Thr56) Antibody
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Immunofluorescence analysis of HUVEC cells, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is blocked with the phospho peptide.
: AO-06-ES5044-50
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