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Antibody Product Overview
| ELK.No | ES5087 |
| Product name | HuB/D rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | ELAVL2; HUB; ELAV-like protein 2; ELAV-like neuronal protein 1; Hu-antigen B; HuB; Nervous system-specific RNA-binding protein Hel-N1; ELAVL4; HUD; PNEM; ELAV-like protein 4; Hu-antigen D; HuD; Paraneoplastic encephalomyelitis antigen HuD |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 1993/1996 |
| Human Swiss-Prot | Q12926/P26378 |
| Source | Rabbit |
| Isotype | IgG |
| Target | HuB/D |
| Fields | |
| Gene name | ELAVL2/ELAVL4 |
| Protein name | ELAV-like protein 2/ELAV-like protein 4 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 15569/15572 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | 432358 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q8CH84/O09032 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ELAV2/4. AA range:61-110 |
| Specificity | HuB/D Polyclonal Antibody detects endogenous levels of HuB/D protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 40kD |
| Background | ELAV like RNA binding protein 2(ELAVL2) Homo sapiens The protein encoded by this gene is a neural-specific RNA-binding protein that is known to bind to several 3' UTRs, including its own and also that of FOS and ID. The encoded protein may recognize a GAAA motif in the RNA. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2010], |
| Function | function:Binds RNA. Seems to recognize a GAAA motif. Can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR.,similarity:Belongs to the RRM elav family.,similarity:Contains 3 RRM (RNA recognition motif) domains.,tissue specificity:Brain; neural-specific., |
| Subcellular location | nucleoplasm, |
| Expression | Brain; neural-specific. |
Additional Images
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Western Blot analysis of Hela cells using HuB/D Polyclonal Antibody diluted at 1:1000
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Immunofluorescence analysis of HepG2 cells, using ELAV2/4 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat and HUVEC cells, using ELAV2/4 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from 293 cells using ELAV2/4 antibody.
: AO-06-ES5087-100
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