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Antibody Product Overview
| ELK.No | ES5099 |
| Product name | APOBEC3A rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | APOBEC3A; Probable DNA dC->dU-editing enzyme APOBEC-3A; Phorbolin-1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 200315 |
| Human Swiss-Prot | P31941 |
| Source | Rabbit |
| Isotype | IgG |
| Target | APOBEC3A |
| Fields | >>Viral life cycle - HIV-1;>>Human immunodeficiency virus 1 infection |
| Gene name | APOBEC3A |
| Protein name | Probable DNA dC->dU-editing enzyme APOBEC-3A |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human APOBEC3A. AA range:27-76 |
| Specificity | APOBEC3A Polyclonal Antibody detects endogenous levels of APOBEC3A protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 26kD |
| Background | This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided b |
| Function | cofactor:Zinc.,function:Lacks cytidine deaminase activity, at least on RNA molecules (monomeric nucleoside substrates or synthetic apoB RNA template). Unable to reduce HIV-1 infectivity in vitro.,miscellaneous:It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22.,similarity:Belongs to the cytidine and deoxycytidylate deaminase family.,tissue specificity:Expressed in peripherical leukocytes and keratinocytes., |
| Subcellular location | Nucleus. Cytoplasm. |
| Expression | Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells. Highly expressed in keratinocytes and in periphery blood monocytes. Also detected in non-lymphoid tissues including lung and adipose tissues. Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia. |
Additional Images
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Western Blot analysis of Jurkat cells using APOBEC3A Polyclonal Antibody
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Western blot analysis of lysates from Jurkat cells, using APOBEC3A Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES5099-100
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