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PIP5KIII (phospho Ser307) rabbit pAb
AO-06-ES5105-50
Antibody Product Overview
| ELK.No | ES5105 |
| Product name | PIP5KIII (phospho Ser307) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | IHC;IF;ELISA |
| Other name | PIKFYVE; KIAA0981; PIP5K3; 1-phosphatidylinositol 3-phosphate 5-kinase; Phosphatidylinositol 3-phosphate 5-kinase; FYVE finger-containing phosphoinositide kinase; PIKfyve; Phosphatidylinositol 3-phosphate 5-kinase type III; PIPkin-III; Type |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 200576 |
| Human Swiss-Prot | Q9Y2I7 |
| Source | Rabbit |
| Isotype | IgG |
| Target | PIP5K |
| Fields | >>Inositol phosphate metabolism;>>Metabolic pathways;>>Phosphatidylinositol signaling system;>>Phagosome;>>Regulation of actin cytoskeleton |
| Gene name | PIKFYVE |
| Protein name | 1-phosphatidylinositol 3-phosphate 5-kinase |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 18711 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9Z1T6 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PIP5K around the phosphorylation site of Ser307. AA range:273-322 |
| Specificity | Phospho-PIP5KIII (S307) Polyclonal Antibody detects endogenous levels of PIP5KIII protein only when phosphorylated at S307. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 237kD |
| Observed band (KD) | |
| Background | Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKf |
| Function | catalytic activity:ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.,disease:Defects in PIKFYVE are the cause of corneal fleck dystrophy (CFD) [MIM:121850]. CFD is an autosomal dominant disorder of the cornea characterized by numerous small white flecks scattered in all levels of the stroma. Although CFD may occasionally cause mild photophobia, patients are typically asymptomatic and have normal vision.,function:Supports the intracellular PIP pool and to a lesser extent, the PI 4,5-P(2) pool. It generates PIP from PI and, to a lesser extent, PI 4,5-P(2) from PI 4-P. There are indications that it phosphorylates the D-5 rather than the D-4 position. Has a role in endosome-related membrane trafficking.,similarity:Contains 1 DEP domain.,similarity:Contains 1 FYVE-type zinc finger.,similarity:Contains 1 PI5K domain.,subcellular location: |
| Subcellular location | Endosome membrane ; Peripheral membrane protein . Early endosome membrane ; Peripheral membrane protein. Cytoplasmic vesicle, phagosome membrane ; Peripheral membrane protein . Late endosome membrane ; Peripheral membrane protein . Mainly associated with membranes of the late endocytic pathway. . |
| Expression | Brain,Epithelium,PCR rescued clones,T-cell, |
Additional Images
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PIP5K (Phospho-Ser307) Antibody
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Immunofluorescence analysis of HeLa cells, using PIP5K (Phospho-Ser307) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain, using PIP5K (Phospho-Ser307) Antibody. The picture on the right is blocked with the phospho peptide.
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: AO-06-ES5105-50
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