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Eps15 (phospho Tyr849) rabbit pAb
AO-06-ES5140-100
Antibody Product Overview
| ELK.No | ES5140 |
| Product name | Eps15 (phospho Tyr849) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | EPS15; AF1P; Epidermal growth factor receptor substrate 15; Protein Eps15; Protein AF-1p |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 2060 |
| Human Swiss-Prot | P42566 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Eps15 |
| Fields | >>Endocytosis |
| Gene name | EPS15 |
| Protein name | Epidermal growth factor receptor substrate 15 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 13858 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P42567 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Eps15 (phospho Tyr849) |
| Specificity | Phospho-Eps15 (Y849) Polyclonal Antibody detects endogenous levels of Eps15 protein only when phosphorylated at Y849. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 100kD |
| Background | This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2009], |
| Function | disease:A chromosomal aberration involving EPS15 is found in acute leukemias. Translocation t(1;11)(p32;q23) with MLL/HRX. The result is a rogue activator protein.,domain:The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.,function:Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits.,PTM:Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis (By similarity). Phosphorylated on serine upon DNA damage, probably by ATM or ATR.,similarity:Contains 2 EF-hand dom |
| Subcellular location | Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, clathrin-coated pit. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes. .; [Isoform 2]: Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Colocalizes with HGS on bilayered clathrin coats on endosomes. |
| Expression | Ubiquitously expressed. |
Additional Images
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Western Blot analysis of JK cells using Phospho-Eps15 (Y849) Polyclonal Antibody
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: AO-06-ES5140-100
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