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Antibody Product Overview
| ELK.No | ES5498 |
| Product name | NARF rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA;IHC |
| Other name | NARF; Nuclear prelamin A recognition factor; Iron-only hydrogenase-like protein 2; IOP2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 26502 |
| Human Swiss-Prot | Q9UHQ1 |
| Source | Rabbit |
| Isotype | IgG |
| Target | NARF |
| Fields | |
| Gene name | NARF |
| Protein name | Nuclear prelamin A recognition factor |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q9CYQ7 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NARF. AA range:262-311 |
| Specificity | NARF Polyclonal Antibody detects endogenous levels of NARF protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 51kD |
| Background | Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It |
| Function | PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the NARF family.,subunit:Interacts with LMNA and binds to the farnesylated C-terminal domain.,tissue specificity:Ubiquitous. Predominantly expressed in skeletal muscle, heart and brain., |
| Subcellular location | Nucleus . |
| Expression | Ubiquitous. Predominantly expressed in skeletal muscle, heart and brain. |
Additional Images
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Western blot analysis of NARF Antibody. The lane on the right is blocked with the NARF peptide.
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Western blot analysis of the lysates from 293 cells using NARF antibody.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES5498-50
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