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BAM32 (phospho Tyr139) rabbit pAb
AO-06-ES5538-50
Antibody Product Overview
| ELK.No | ES5538 |
| Product name | BAM32 (phospho Tyr139) rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | DAPP1; BAM32; HSPC066; Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide; hDAPP1; B lymphocyte adapter protein Bam32; B-cell adapter molecule of 32 kDa |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 27071 |
| Human Swiss-Prot | Q9UN19 |
| Source | Rabbit |
| Isotype | IgG |
| Target | DAPP1 |
| Fields | >>B cell receptor signaling pathway |
| Gene name | DAPP1 |
| Protein name | Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 26377 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9QXT1 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DAPP1 around the phosphorylation site of Tyr139. AA range:105-154 |
| Specificity | Phospho-BAM32 (Y139) Polyclonal Antibody detects endogenous levels of BAM32 protein only when phosphorylated at Y139. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 32kD |
| Background | function:May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.,induction:Upon B-cell activation.,PTM:Phosphorylated on tyrosine residues.,similarity:Contains 1 PH domain.,similarity:Contains 1 SH2 domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation.,subunit:Interacts with PtdIns(3,4,5)P3 and PLCG2. In vitro, interacts with PtdIns(3,4)P2.,tissue specificity:Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells., |
| Function | function:May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.,induction:Upon B-cell activation.,PTM:Phosphorylated on tyrosine residues.,similarity:Contains 1 PH domain.,similarity:Contains 1 SH2 domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation.,subunit:Interacts with PtdIns(3,4,5)P3 and PLCG2. In vitro, interacts with PtdIns(3,4)P2.,tissue specificity:Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells., |
| Subcellular location | Cytoplasm . Membrane ; Peripheral membrane protein . Membrane-associated after cell stimulation leading to its translocation. |
| Expression | Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells. |
Additional Images
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Western Blot analysis of 293 cells using Phospho-BAM32 (Y139) Polyclonal Antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DAPP1 (Phospho-Tyr139) Antibody
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Immunofluorescence analysis of A549 cells, using DAPP1 (Phospho-Tyr139) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from 293 cells treated with Insulin 0.01U/ml 2', using DAPP1 (Phospho-Tyr139) Antibody. The lane on the right is blocked with the phospho peptide.
: AO-06-ES5538-50
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