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Antibody Product Overview
| ELK.No | ES5546 |
| Product name | Arfaptin 1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | ARFIP1; Arfaptin-1; ADP-ribosylation factor-interacting protein 1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 27236 |
| Human Swiss-Prot | P53367 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Arfaptin 1 |
| Fields | |
| Gene name | ARFIP1 |
| Protein name | Arfaptin-1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | 60382 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q9JHU5 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ARFIP1. AA range:271-320 |
| Specificity | Arfaptin 1 Polyclonal Antibody detects endogenous levels of Arfaptin 1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 40kD |
| Background | function:Putative target protein of ADP-ribosylation factor.,similarity:Contains 1 AH domain.,subunit:Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1. Binds with lower affinity to ARF5 and with very little affinity to ARF6.,tissue specificity:Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart., |
| Function | function:Putative target protein of ADP-ribosylation factor.,similarity:Contains 1 AH domain.,subunit:Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1. Binds with lower affinity to ARF5 and with very little affinity to ARF6.,tissue specificity:Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart., |
| Subcellular location | Golgi apparatus . Golgi apparatus, trans-Golgi network membrane . |
| Expression | Ubiquitously expressed (PubMed:9038142). Higher levels in liver, pancreas, placenta, skeletal muscle and heart (PubMed:9038142). |
Additional Images
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Immunohistochemical analysis of paraffin-embedded Human thymus gland. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of A549 cells, using ARFIP1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat cells, using ARFIP1 Antibody. The lane on the right is blocked with the synthesized peptide.
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: AO-06-ES5546-100
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