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Antibody Product Overview
| ELK.No | ES5582 |
| Product name | GPR133 rabbit pAb |
| Reactivity | Human;Monkey |
| Applications | WB;IF;ELISA |
| Other name | GPR133; PGR25; Probable G-protein coupled receptor 133; G-protein coupled receptor PGR25 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 283383 |
| Human Swiss-Prot | Q6QNK2 |
| Source | Rabbit |
| Isotype | IgG |
| Target | GPR133 |
| Fields | |
| Gene name | GPR133 |
| Protein name | Probable G-protein coupled receptor 133 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q80T32 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GPR133. AA range:461-510 |
| Specificity | GPR133 Polyclonal Antibody detects endogenous levels of GPR133 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 96kD |
| Background | The adhesion G-protein-coupled receptors (GPCRs), including GPR133, are membrane-bound proteins with long N termini containing multiple domains. GPCRs, or GPRs, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins (summary by Bjarnadottir et al., 2004 [PubMed 15203201]).[supplied by OMIM, Nov 2010], |
| Function | function:Orphan receptor.,similarity:Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.,similarity:Contains 1 GPS domain., |
| Subcellular location | Cell membrane ; Multi-pass membrane protein . |
| Expression | Up-regulated in CD133(+) cell population of glioblastoma. |
Additional Images
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Immunofluorescence analysis of HeLa cells, using GPR133 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COS7 cells, using GPR133 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from K562 cells using GPR133 antibody.
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: AO-06-ES5582-50
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