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HMG-14 (phospho Ser21) rabbit pAb
AO-06-ES5743-100
Antibody Product Overview
| ELK.No | ES5743 |
| Product name | HMG-14 (phospho Ser21) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | IHC;IF;ELISA |
| Other name | HMGN1; HMG14; Non-histone chromosomal protein HMG-14; High mobility group nucleosome-binding domain-containing protein 1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 3150 |
| Human Swiss-Prot | P05114 |
| Source | Rabbit |
| Isotype | IgG |
| Target | HMG-14 |
| Fields | |
| Gene name | HMGN1 |
| Protein name | Non-histone chromosomal protein HMG-14 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P18608 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HMG14 around the phosphorylation site of Ser21. AA range:10-59 |
| Specificity | Phospho-HMG-14 (S21) Polyclonal Antibody detects endogenous levels of HMG-14 protein only when phosphorylated at S21. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 11kD |
| Observed band (KD) | |
| Background | The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011], |
| Function | function:Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.,mass spectrometry: PubMed:10739259,PTM:Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.,RNA editing:Partially edited. A new initiator methionine may be created by a single uridine insertion in the 5'-UTR, causing an N-terminal extension of 45 amino acids. The existence of the RNA edited version is supported by direct protein sequencing by MS/MS of the following peptides specific to that version: 23-31 and 40-48. The RNA edited version is |
| Subcellular location | Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus. |
| Expression | Bone marrow,Brain,Epithelium,Liver,Ovary,Pooled,Skin, |
Additional Images
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HMG14 (Phospho-Ser21) Antibody
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Immunofluorescence analysis of COS7 cells, using HMG14 (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HMG14 (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.
: AO-06-ES5743-100
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