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Antibody Product Overview
| ELK.No | ES5773 |
| Product name | E-Ras rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IF;ELISA |
| Other name | ERAS; HRAS2; HRASP; GTPase ERas; E-Ras; Embryonic stem cell-expressed Ras |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3266 |
| Human Swiss-Prot | Q7Z444 |
| Source | Rabbit |
| Isotype | IgG |
| Target | E-Ras |
| Fields | |
| Gene name | ERAS |
| Protein name | GTPase Eras |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q7TN89 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ERAS. AA range:184-233 |
| Specificity | E-Ras Polyclonal Antibody detects endogenous levels of E-Ras protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 25kD |
| Background | This gene encodes a constitutively active member of the small GTPase Ras protein family. The encoded protein activates the phosphatidylinositol 3-kinase signal transduction pathway in undifferentiated stem cells, but is not expressed in differentiated cells. This gene may be involved in cancer and chemotherapy resistance. [provided by RefSeq, Dec 2012], |
| Function | enzyme regulation:Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).,function:Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells.,similarity:Belongs to the small GTPase superfamily. Ras family.,subunit:Interacts with PIK3CD., |
| Subcellular location | Cell membrane ; Lipid-anchor ; Cytoplasmic side . |
| Expression | Brain, |
Additional Images
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Immunofluorescence analysis of A549 cells, using ERAS Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat cells, using ERAS Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HeLa cells using ERAS antibody.
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: AO-06-ES5773-50
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