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IL-7R (phospho Tyr449) rabbit pAb
AO-06-ES5884-100
Antibody Product Overview
| ELK.No | ES5884 |
| Product name | IL-7R (phospho Tyr449) rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IF;ELISA |
| Other name | IL7R; Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CDw127; CD antigen CD127 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 3575 |
| Human Swiss-Prot | P16871 |
| Source | Rabbit |
| Isotype | IgG |
| Target | IL-7R |
| Fields | >>Cytokine-cytokine receptor interaction;>>FoxO signaling pathway;>>PI3K-Akt signaling pathway;>>JAK-STAT signaling pathway;>>Hematopoietic cell lineage;>>Pathways in cancer;>>Primary immunodeficiency |
| Gene name | IL7R |
| Protein name | Interleukin-7 receptor subunit alpha |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 16197 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P16872 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IL-7R/CD127 around the phosphorylation site of Tyr449. AA range:410-459 |
| Specificity | Phospho-IL-7R (Y449) Polyclonal Antibody detects endogenous levels of IL-7R protein only when phosphorylated at Y449. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 60kD |
| Background | The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found. [provided by RefSeq, Dec 2015], |
| Function | disease:A genetic variation in transmembrane domain of IL7R is associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. Overtransmission of the major 'C' allele coding for Thr-244 are detected in offspring affected with multiple sclerosis. In vitro analysis of transcripts from minigenes containing either 'C' allele (Thr-244) or 'T' allele (Ile-244) shows that the 'C' allele results in an approximately two-fold increase in the skipping of exon 6, leading to increased production of a soluble form of IL7R. Thus, the multiple sclerosis associated 'C' risk allele of IL7R would probably decrease membrane-bound expression of IL7R. As this risk allele is common in the general population, some additional triggers are probably required for the development and progression of MS.,disease:Defects in IL7R are a cause of autosomal recessive severe combined immunodeficiency T-cell-negativ |
| Subcellular location | [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Cell membrane; Single-pass type I membrane protein.; [Isoform 4]: Secreted. |
| Expression | B-cell,Epithelium,Spleen,Testis, |
Additional Images
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Immunofluorescence analysis of HUVEC cells, using IL-7R/CD127 (Phospho-Tyr449) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from COLO205 cells, using IL-7R/CD127 (Phospho-Tyr449) Antibody. The lane on the right is blocked with the phospho peptide.
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