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AR (phospho Tyr363) rabbit pAb
AO-06-ES5936-50
Antibody Product Overview
| ELK.No | ES5936 |
| Product name | AR (phospho Tyr363) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | AR; DHTR; NR3C4; Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 367 |
| Human Swiss-Prot | P10275 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Androgen Receptor |
| Fields | >>Oocyte meiosis;>>Pathways in cancer;>>Chemical carcinogenesis - receptor activation;>>Prostate cancer |
| Gene name | AR |
| Protein name | Androgen receptor |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 11835 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P19091 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24208 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P15207 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Androgen Receptor around the phosphorylation site of Tyr363. AA range:331-380 |
| Specificity | Phospho-AR (Y363) Polyclonal Antibody detects endogenous levels of AR protein only when phosphorylated at Y363. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 85kD |
| Background | The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoform |
| Function | disease:Defects in AR are the cause of androgen insensitivity syndrome (AIS) [MIM:300068]; previously known as testicular feminization syndrome (TFM). AIS is an X-linked recessive form of pseudohermaphroditism due end-organ resistance to androgen. Affected males have female external genitalia, female breast development, blind vagina, absent uterus and female adnexa, and abdominal or inguinal testes, despite a normal 46,XY karyotype.,disease:Defects in AR are the cause of androgen insensitivity syndrome partial (PAIS) [MIM:312300]; also known as Reifenstein syndrome. PAIS is characterized by hypospadias, hypogonadism, gynecomastia, genital ambiguity, normal XY karyotype, and a pedigree pattern consistent with X-linked recessive inheritance. Some patients present azoospermia or severe oligospermia without other clinical manifestations.,disease:Defects in AR are the cause of spinal and bulb |
| Subcellular location | Nucleus . Cytoplasm . Detected at the promoter of target genes (PubMed:25091737). Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1. . |
| Expression | [Isoform 2]: Mainly expressed in heart and skeletal muscle. ; [Isoform 3]: Expressed in basal and stromal cells of the prostate (at protein level). |
Additional Images
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Western blot analysis of lysates from Jurkat cells treated with UV 15', using Androgen Receptor (Phospho-Tyr363) Antibody. The lane on the right is blocked with the phospho peptide.
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: AO-06-ES5936-50
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